This algorithm is used to help diagnose HIV-1 and HIV-2 infection, including acute infection, and to differentiate HIV-1 from HIV-2. It is consistent with the HIV diagnostic algorithm recommended by the Centers for Disease Control and Prevention [2]. It can be used in adults, including pregnant women, and in children 2 years or older. This panel begins with an antigen/antibody assay that allows for detection of acute HIV-1 infection based on the presence of p24 antigen before seroconversion occurs, and HIV-1 and HIV-2 antibodies post-seroconversion. If the HIV-1/HIV-2 antigen/antibody test is positive, an antibody-differentiation test is done (at additional charge) to confirm infection and identify the presence of HIV-1 or HIV-2 specific antibodies. However, the HIV-1/HIV-2 differentiation test can be negative during acute infection (prior to development of specific antibodies). If the antibody-based HIV-1/HIV-2 supplemental test is negative, the specimen will be reflexed to the HIV-1
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